Single molecule imaging by tapping mode AFM under aqueous conditions

Tapping Mode AFM Imaging for Functionalized Surfaces

Mechanisms of single-walled carbon nanotube probe-sample multistability in tapping mode AFM imaging.

When using single-walled carbon nanotube (SWNT) probes to create AFM images of SWNT samples in tapping mode, elastic deformations of the probe and sample result in a decrease in the apparent width of the sample. Here we show that there are two major mechanisms for this effect, smooth gliding and snapping, and compare their dynamics to the case when a conventional silicon tip is used to image a ...

AFM-Tip Functionalization for Single Molecule Recognition Imaging

The high force sensitivity of the atomic force microscope (AFM) has opened the possibility of measuring interand intramolecular forces of biomolecules on the single molecule level. Covalent binding of bioligands to AFM tips converts them into monomolecular biosensors by which cognate receptors can be localized on the sample surface and fine details of ligand-receptor interaction can be studied....

A Study of Tapping Mode Trajectories in an AFM

Abstract In an effort to aid in the understanding of the nonlinear dynamics of the trajectories followed by an Atomic Force Microscope (AFM), I have written an interactive java applet simulator using a simple inelastic billiard model developed by Cvitanović and Paškauskas. We study the trajectories followed by the tip of the AFM as a function of its physical parameters and initial conditions. T...

Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM.

The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but non-invasive attachment ...